Stem Cell Laboratory
Stem Cell laboratory was established in Shiraz Institute for Cancer Research (ICR), Shiraz University of Medical Sciences in July 2007, and the first adipose derived mesenchymal stem cells were isolated and cultured from adipose tissue of a patient with breast carcinoma.
Stem cells are defined as pluripotent progenitor cells with self-renewal capacity and the ability to differentiate to multiple cell lineages. Adult stem cells are stem cells that can be derived from different parts of the body such as bone marrow, heart, abdomen, cord blood, breast, prostate, Wharton jelly and brain. These cells have the extraordinary capacity to generate several differentiated cell types such as bone, muscle, hepatocytes, neuron and fat whilst self-renewing themselves. The best-characterized and most studied adult stem cells are the hematopoietic stem cells (HSCs) and the mesenchymal stem cells (MSCs). MSCs can be isolated from adipose tissue and bone marrow but they can be harvested by a minimally invasive procedure from adipose tissue. These properties have generated tremendous interest in the potential use of MSCs for regenerative medicine. The mission of the stem cell laboratory is divided into two parts:
1-The effects of MSCs on tumor microenvironment especially on different kinds of immune cells
2- Application of MSCs in regenerative medicine
Based on explanations, the current techniques used in this lab. are as follows:
Isolation of different kinds of stem cells , differentiation of MSCs with or without using scaffold, study the effects of MSCs on immune cells and cancer cells using coculture method, proliferation by CFSE, flowcytometry method, gene transfection ,would healing assay, MSCs supernatants for using in animal models, qRT-PCR method, MTT assay, western blot, and Elisopot assay.
Publication
1- Razmkhah M, Jaberipour M, Erfani N, Habibagahi M, Talei AR, Ghaderi A. Adipose derived stem cells (ASCs) isolated from breast cancer tissue express IL-4, IL-10 and TGF-β1 and upregulate expression of regulatory molecules on T cells: do they protect breast cancer cells from the immune response? Cellular Immunology. 2011;266(2):116-22.
2- Fazel Anvari-Yazdi A, Tahermanesh K, Hadavi SM, Talaei-Khozani T, Razmkhah M, Abed SM, Mohtasebi MS. Cytotoxicity assessment of adipose-derived mesenchymal stem cells on synthesized biodegradable Mg-Zn-Ca alloys. Materials Science and Engineering: C (Materials for Biological Applications). 2016;69:584-97.
3- Yazdani Y, Sharifi Rad MR, Taghipour M, Chenari N, Ghaderi A, Razmkhah M. Genistein suppression of matrix metalloproteinase 2 (MMP-2) and vascular endothelial growth factor (VEGF) expression in mesenchymal stem cell like cells isolated from high and low grade gliomas. Asian Pacific Journal of Cancer Prevention. 2016;17(12):5303-307.
4- Ayatollahi M, Talaei-Khozani T, Razmkhah M. Growth suppression effect of human mesenchymal stem cells from bone marrow, adipose tissue, and Wharton's jelly of umbilical cord on PBMCs. Iranian Journal of Basic Medical Science. 2016;19(2):145-53.
5- Razmkhah M, Mansourabadi Z, Mohtasebi MS, Talei AR, Ghaderi A. Cancer and normal adipose-derived mesenchymal stem cells (ASCs): Do they have differential effects on tumor and immune cells? Cell Biology International. 2018; 42(3):334-43.
6- Razmkhah M, Abtahi S, Ghaderi A. Mesenchymal stem cells, immune cells and tumor cells crosstalk: a sinister triangle in the tumor microenvironment. Current Stem Cell Research and Therapy. 2019;14(1):43-51.
7- Fakhimi M, Talei AR, Ghaderi A, Habibagahi M, Razmkhah M. Helios, CD73 and CD39 induction in regulatory T cells exposed to adipose derived mesenchymal stem cells. Cell Journal. 2020 22(2):236-44.
Projects
1-Assessment of the effect of gamma radiation on the proliferation, angiogenesis and immune suppressive characteristics of adipose derived mesenchymal stem cells and the role of these stem cells in the expression of radiation resistance genes in cancer cell lines.
2- IDO gene suppression in Adipose Derived mesenchymal Stem Cells (ASCs) and its effects on the phenotypic changes of T lymphocytes and invasion properties of breast cancer cell lines.
3- Comparative study of immunomodulatory properties of cancer and normal adipose tissue-derived mesenchymal stem cells (ASCs) isolated from patients with breast cancer.
4- PD-L1 receptor suppression in breast tumor cells and concomitant activation of T lymphocytes with bispecific T-cell engager (BiTE ) releasing adipose-derived mesenchymal stem cells (ASCs).
5- Establishment and characterization of a new human tongue carcinoma cell line.
6-The effect of adipose drives mesenchymal stem cells loaded with nanoliposomes containing mineral pitch seeded into special scaffold on bone defects repair in an animal model.
